Blu10 Plus Prestained Protein Ladder BLUltra Prestained

Description

The Blu10 Plus Prestained Protein Ladder (BLUltra) is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. The Blu10 Plus Prestained Protein Ladder (BLUltra) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

-Guide for Molecular Weight Estimation (kDa)

Migration patterns of Blu10 Plus Prestained Protein Ladder (BLUltra) in different electrophoresis conditions are listed below :

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. That’s why we’re pleased to launch the color-enhanced Blu10 Plus (BLUltra) prestained protein standards.

As you may see the comparison data shown below, Blu10 Plus (BLUltra) shows a better resolution than the Blu12 (BLUeye) & Blu10 (BlueRAY) for the protein bands from 95 kDa to 270 kDa.

Lane 1: PMU12-0500 UNveil Unstained Protein Ladder (Bio-Helix) / 5μl
Lane 2: PMB01-0500 / PM001-0500 Blu10 Plus Prestained Protein Ladder (BLUltra) (Bio-Helix) / 5μl
Lane 3: Bio-Rad 161-0374 Precision Plus Protein™ Dual Color Standards / 5μl
Lane 4: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 5μl
Lane 5: PMB12-0500 / PM007-0500 Blu12 Prestained Protein Ladder (BLUeye) (Bio-Helix) / 5μl
Lane 6: PMU12-0500 UNveil Unstained Protein Ladder (Bio-Helix) / 10μl
Lane 7: PMB01-0500 / PM001-0500 Blu10 Plus Prestained Protein Ladder (BLUltra) (Bio-Helix) / 10μl
Lane 8: Bio-Rad 161-0374 Precision Plus Protein™ Dual Color Standards / 10μl
Lane 9: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 10μl
Lane 10: PMB12-0500 / PM007-0500 Blu12 Prestained Protein Ladder (BLUeye) (Bio-Helix) / 10μl

Required materials but not provided:

• Vertical Electrophoresis system
• Power supplies
• Vortex or equivalent
• Microcentrifuge