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Product
100 mM dNTP Set
BIO-HELIX – DN001-0250
SIZE: 4 x 250 μl
Bio-Helix 100 mM dNTP (2′-deoxynucleoside 5′-triphosphate) Set consists of all four deoxynucleotides (dATP, dCTP, dGTP, dTTP), each at a concentration of 100 mM. The deoxynucleotides are suitable for use in polymerase chain reaction (PCR), sequencing, fill-in, nick translation, cDNA synthesis, and TdT tailing reactions. The product is supplied as ready-to-use solutions.
The 100 mM dNTP Set includes 100 mM each of dATP, dCTP, dGTP, and dTTP.
Each vial contains 250 μl of 25 μmol dNTP in purified water (pH 7.5).
Volume And Concentration
20 μl Final Reaction Volume
| Final dNTP Conc | dNTP Volume |
| 0.2 mM | 0.04 μl |
| 0.5 mM | 0.1 μl |
| 1.0 mM | 0.2 μl |
| 1.5 mM | 0.3 μl |
25 μl Final Reaction Volume
| Final dNTP Conc | dNTP Volume |
| 0.2 mM | 0.05 μl |
| 0.5 mM | 0.125 μl |
| 1.0 mM | 0.25 μl |
| 1.5 mM | 0.375 μl |
50 μl Final Reaction Volume
| Final dNTP Conc | dNTP Volume |
| 0.2 mM | 0.1 μl |
| 0.5 mM | 0.25 μl |
| 1.0 mM | 0.5 μl |
| 1.5 mM | 0.75 μl |
