Link Biotech
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VERO HCPs Elisa Kit

SKU Code LBFN-028MK
Species Monkey
Sensitivity 0.188ng/ml
Detection Range 0.313-20ng/ml
Size 96 T | 48 T
Price POR
SKU: LBFN-028MK
VERO HCPs (VERO host cell proteins) ELISA Kit - Details
Alternative Names Vero HCPs ELISA Kit, VERO host cell proteins ELISA Kit
Sample Type Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method Sandwich ELISA, Double Antibody
Detection Wavelength OD450
Reaction Duration 4 hours
Storage 2-8°C(Sealed), Don't cryopreserve.
Specificity Specifically binds with Vero HCPs , no obvious cross reaction with other analogues.

Additional information

Sensitivity

0.188ng/mL

Detection-Range

0.313-20ng/ml

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Vero HCPs antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Vero HCPs bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Vero HCPs in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.