| Vasopressin ELISA Kit | |
| UniProt ID | P01185 |
| Sample Volume | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Storage | 2-8°C(Sealed), Don't cryopreserve. |
| Specificity | Specifically binds with Vasopressin , no obvious cross reaction with other analogues. |
A-1/264A Paschim Vihar, New Delhi - 110063
Vasopressin ELISA Kit
| SKU Code | LBEK-016U |
| Species | Universal |
| Sensitivity | 0.188pg/ml |
| Detection-range | 0.313-20pg/ml |
| Size | 96 T | 48 T |
| Price | POR |
| Brand | Link Biotech |
- Product Specification
- Additional information
- Standard Curve
- Recovery
- Linearity
- Precision(%)
- Stability
Additional information
| sensitivity | 0.188pg/ml |
|---|---|
| detection-range | 0.313-20pg/ml |
| Brand | Link Biotech |
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
| STD.(pg/ml) | OD-1 | OD-2 | Average |
|---|---|---|---|
| 0 | 2.135 | 2.244 | 2.179 |
| 0.312 | 1.671 | 1.757 | 1.706 |
| 0.625 | 1.444 | 1.518 | 1.474 |
| 1.25 | 1.198 | 1.259 | 1.223 |
| 2.5 | 1.021 | 1.073 | 1.042 |
| 5 | 0.683 | 0.717 | 0.697 |
| 10 | 0.47 | 0.497 | 0.483 |
| 20 | 0.257 | 0.27 | 0.263 |
Add a certain amount of Vasopressin into the sample. Calculate the recovery by comparing the measured value with the expected amount of Vasopressin in the sample.
| Sample Type | Recovery Range(%) | Average(%) |
|---|---|---|
| serum(n=10) | 87-105 | 92 |
| EDTA plasma(n=10) | 88-102 | 94 |
| Heparin plasma(n=10) | 88-104 | 98 |
Dilute the sample with a certain amount of Vasopressin at 1:2, 1:4 and 1:8 to get the recovery range.
| Sample Type | 1:2 | 1:4 | 1:8 |
|---|---|---|---|
| serum(n=10) | 94-101% | 86-101% | 91-99% |
| EDTA plasma(n=10) | 82-94% | 87-98% | 82-91% |
| Heparin plasma(n=10) | 82-98% | 82-95% | 90-96% |
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
| Item | Intra-assay Precision | Inter-assay Precision | ||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/ml) | 0.62 | 2.59 | 10.37 | 0.6 | 2.43 | 9.96 |
| Standard deviation | 0.04 | 0.14 | 0.33 | 0.03 | 0.11 | 0.54 |
| CV(%) | 6.42 | 5.23 | 3.21 | 4.88 | 4.68 | 5.43 |
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
| ELISA kit(n=5) | 37°C for 1 month | 2-8°C for 6 months | 2-8°C for 12 months |
| Average(%) | 80 | 95-100 | 85-98 |