The UltraScence Atto Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is the most sensitive and brightest ECL Western Substrate among our UltraScence product lines for low-femtogram to high-attogram detection of antigen with excellent sensitivity and long signal duration. UltraScence Atto Western Substrate is compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal.


– No optimization required.
Switching to the UltraScence Atto Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ West Atto, does not require optimization or protocol changes.
– High degree of sensitivity and enhanced chemiluminescence duration.
UltraScence Atto Western Substrate enables an accurate low-femtogram to high-attogram detection of protein on the same immunoblot after a single exposure.
– Optimized for use with PVDF and nitrocellulose membranes.
– Compatible with Western Blotting Markers.
– Optimized for film- and CCD-based imaging.
– Patent pending product.