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Recombinant Human CD274 - Specification |
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Uniprot ID |
Q9NZQ7 |
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Tags |
C-terminal His Tag |
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Mol. Weight |
35-38 kDa |
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Expression Region |
19-239 |
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Purity |
Greater than 90% as determined by SDS-PAGE. |
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Activity |
Measured by its binding ability in a functional ELISA. Immobilized human CD274 at 20 μg/mL (100 μl/well) can bind human PD1 with a linear range of 0.05-0.1 μg/mL. |
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Formulation |
Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4. |
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Reconstitution |
Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex. |
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Storage |
The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions. Avoid repeated freeze/thaw cycle. |
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Alternative Names |
B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1 |
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