Link Biotech

Elisa-Kit

Rat IL-6(Interleukin 6) ELISA Kit

SKU Code LBRB-012R
Species Rat
Sensitivity 28.77 pg/mL
Detection Range 31.25- 2000pg/mL
Size 96 T | 48 T
Price $ 365 | $ 265
SKU: LBRB-012R
Categories: ,

Description

Rat IL-6(Interleukin 6) ELISA Kit - Product Details
Uniprot ID P20607
Standard 2000pg/mL
Sample type Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL
Reaction time 3.5H
Research Area Tumor immunity;Endocrinology;Hormone metabolism
Alternative Names
IL-6;IL 6;IL6;Interleukin-6;Interleukin 6;Interleukin6;B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor , HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A;Hybridoma growth factor;Interferon beta-2;IFNB 2;IFNB-2;IFN-B2
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-6. You can calculate the concentration of Rat IL-6 in the samples by comparing the OD of the samples to the standard curve.

Additional information

Sensitivity

28.77 pg/mL
Detection Range

31.25- 2000pg/mL

Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL) OD Corrected
2000.00 2.392 2.340
1000.00 1.745 1.693
500.00 0.873 0.821
250.00 0.473 0.421
125.00 0.264 0.212
62.50 0.131 0.079
31.25 0.095 0.043
0.00 0.052 0.000

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 13.51 191.69 357.43 14.35 164.67 355.13
Standard deviation 1.19 7.21 17.16 1.00 8.04 10.81
C V (%) 7.40 5.55 4.79 7.54 3.80 6.16

Rate of Recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Avg Recovery(%)
Serum (n=8) 83-97 90
EDTA plasma (n=8) 87-95 91
Cell culture media (n=8) 81-95 88

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 93-102 86-96 87-96
Average (%) 96 95 92
1:4 Range (%) 81-96 93-104 85-97
Average (%) 89 98 96
1:8 Range (%) 93-106 89-97 85-94
Average (%) 96 93 88
1:16 Range (%) 82-95 85-94 87-96
Average (%) 88 93 93

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