Description
| Alternative Names: | 4-HNE, HNE, 4-hydroxy-2-nonenal, 4-Hydroxynonenal | |||||||||||||||||||||||||||
| Catalogue No. | 3738ELK | |||||||||||||||||||||||||||
| Size | 96T | |||||||||||||||||||||||||||
| Reactivity | Rat | |||||||||||||||||||||||||||
| Range | 15.63-1000 pg/mL | |||||||||||||||||||||||||||
| Sensitivity | 7.54 pg/mL | |||||||||||||||||||||||||||
| Assay Type | Competitive Inhibition | |||||||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates | |||||||||||||||||||||||||||
| Assay Length | 2h | |||||||||||||||||||||||||||
| Research Area | Signal transduction; Metabolic pathway; Infection immunity; Hormone metabolism; Dermatology; | |||||||||||||||||||||||||||
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HNE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HNE. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HNE in the samples is then determined by comparing the OD of the samples to the standard curve. | |||||||||||||||||||||||||||
| Standard Curve | 
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| Precision | Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. |
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| Recovery | Matrices listed below were spiked with certain level of recombinant 4-HNE and the recovery rates were calculated by comparing the measured value to the expected amount of 4-HNE in samples.
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| Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 4-HNE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| Note | For Research Use Only |
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