Description
| Product Name | Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb |
| Catalogue No. | 7G9-2801022E |
| Gene Name | H2AX |
| Alternative Names | H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X |
| Swiss Prot ID | P16104 |
| Gene ID | 3014 |
| Background | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. |
| Research Field | Epigenetics and Nuclear Signalling |
| Product Categories: | Primary Antibody |
| Host | Mouse |
| Reactivity | Human ,Mouse |
| Purification | Immunogen affinity purified |
| Application | WB,IHC,ICC/IF,FLOW |
| Dilution | WB: 1/500-1/1000 IF: 1/50-1/200 |
| Observed MW | Calculated MW: 15 kDa; Observed MW: 15 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Clonality | Monoclonal Antibody |
| Clonality No | 7G9-H4 |
| Isotype | IgG1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X. |
| Purification: | Affinity Purified |
| Conjugation | Unconjugated |
| Modification | Phosphorylated |
| Form: | Liquid |
| Concentration: | see label |
| Buffer System: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Western blot analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyureatreated 3T3 lysates using Phospho-Histone H2A.X (Ser139) antibody (E2201082-7G9).
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| Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (7G9) in 3T3 or Hydroxyureatreated 3T3 using Phospho-Histone H2A.X (Ser139) antibody (E2201082-7G9).
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