PE Anti-Mouse CD45.2 Antibody

SKU Code LBFN-007FC
Host Armenian Hamster
Reactivity Mouse
Tested Application FC
Size 25μg | 100μg
Price POR
SKU: LBFN-006FC

Description

PerCP-Cy5.5 Anti-Mouse TCRβ Antibody(H57-597) - Specifications
Synonyms TCR-ββ-TCR| TCR-β chain antibody
Isotype IgG
Form Liquid
Concentration 0.2mg/mL
Conjugation PerCP-Cy5.5
Conjugation Information PerCP-Cy5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm
Clonality Monoclonal
Clone ID H57-597
Storage PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Additional information

Host

Mouse

Reactivity

Mouse

Tested Application

FC

Background

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1μg/10⁶ cells in 100 μL volume].

Validated Images

C57BL/6 murine splenocytes are stained with PE Anti-Mouse CD45.2 Antibody

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