Link Biotech

Elisa-Kit

Mouse ADP/Acrp30(Adiponectin) Elisa Kit

SKU Code LBRB-016H
Species Human
Sensitivity 9.38 pg/mL
Detection Range 15.63-1000pg/mL
Size 96 T | 48 T
Price $ 365 | $ 265
SKU: LBRB-016H
Categories: ,

Description

Mouse ADP/Acrp30(Adiponectin) ELISA Kit - Product Details
Alternative Names Acrp30, GBP28, ACDC, APM1, ADPN, AdipoQ, ADIPQTL1
Uniprot ID Q60994
Standard 1000pg/mL
Sample type Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL
Reaction time 3.5H
Research Area Metabolic pathway;Infection immunity
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ADP/Acrp30. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ADP/Acrp30. You can calculate the concentration of Mouse ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve.

Additional information

Sensitivity

9.38 pg/mL
Detection Range

15.63-1000pg/mL

Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL) OD Corrected
1000.00 2.451 2.430
500.00 1.571 1.550
250.00 0.878 0.857
125.00 0.443 0.422
62.50 0.234 0.213
31.25 0.128 0.107
15.63 0.077 0.056
0.00 0.021 0.000

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean(pg/mL) 25.39 137.86 256.43 22.90 258.18 359.64
Standard deviation 1.16 9.30 19.73 1.25 7.20 14.35
C V (%) 5.91 2.87 5.65 7.41 7.73 7.54

Rate of Recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range(%) Avg Recovery(%)
Serum (n=8) 85-97 91
EDTA plasma (n=8) 83-99 95
Cell culture media (n=8) 85-97 91

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
1:2 Range (%) 95-102 89-97 89-102
Average (%) 102 90 92
1:4 Range (%) 85-96 85-92 97-103
Average (%) 89 87 101
1:8 Range (%) 90-98 95-103 88-96
Average (%) 96 101 92
1:16 Range (%) 82-93 87-93 95-101
Average (%) 85 92 97

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