Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

MF-Mouse PD-L1 ELISA Kit

SKU Code LBEK-0030M
Species Mouse
Sensitivity 1.88 pg/mL
Detection Range 3.13-200pg/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-0030M
MF-Mouse PD-L1(Programmed Cell Death Protein 1 Ligand 1) ELISA Kit
Uniprot ID Q9EP73
Detection Method Sandwich
Standard 200pg/mL
Reaction time 3hours
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=25μL
Research Area Cardiovascular, Cancer, Immunology
Alternative Names Avelumab, B7-H, B7H1, B7-H1, B7H1PDCD1L1, CD274 antigenMGC142294, CD274 molecule, CD274, PDCD1L1, PDCD1LG1, PDCD1LG1MGC142296, PDL1, PD-L1, PD-L1B7 homolog 1, PDL1PDCD1 ligand 1, programmed cell death 1 ligand 1, Programmed death ligand 1
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PD-L1. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Mouse PD-L1. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PD-L1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse PD-L1. You can calculate the concentration of Mouse PD-L1 in the samples by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

1.88 pg/mL

detection-range

3.13-200pg/mL

brand

Link Biotech