Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

MF-Human OPG ELISA Kit

SKU Code LBEK-0082H
Species Human
Sensitivity 37.5 pg/mL
Detection Range 62.5-4000pg/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-0082H
MF-Human OPG(Osteoprotegerin) ELISA Kit
Uniprot ID O00300
Detection Method Sandwich
Standard 10ng/mL
Reaction time 3hours
Sample Type Serum, Plasma and Saliva and Other biological samples;Sample volume:25μL
Research Area Cancer, Immunology
Alternative Names TNFRSF11B , OCIF, TR1
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OPG. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human OPG. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human OPG. You can calculate the concentration of Human OPG in the samples by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

0.1 ng/mL

detection-range

0.16-10ng/mL

brand

Link Biotech