MF-Human GHR ELISA Kit

MF-Human GHR(Growth Hormone Receptor) ELISA Kit
Uniprot ID	P10912
Detection Method	Sandwich
Standard	100ng/mL
Reaction time	3hours
Sample Type	Tissue homogenate and Cell lysate; Sample volume：25μL
Research Area	Cell biology, Cancer, Developmental Biology;
Alternative Names	GH receptor, Somatotropin receptor
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GHR. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human GHR. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GHR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human GHR. You can calculate the concentration of Human GHR in the samples by comparing the OD of the samples to the standard curve.

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