MF-Human EGFR2(Epidermal Growth Factor Receptor 2) ELISA Kit

MF-Human EGFR2(Epidermal Growth Factor Receptor 2) ELISA Kit
Uniprot ID	P04626
Detection Method	Sandwich
Standard	2000pg/mL
Reaction time	3hours
Sample Type	Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL
Research Area	Signaling transduction, Cancer, Immunology
Alternative Names	ERBB2, HER2, CD34, p185, MLN 19, NEU
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human EGFR2. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human EGFR2. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EGFR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human EGFR2. You can calculate the concentration of Human EGFR2 in the samples by comparing the OD of the samples to the standard curve.

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