Human REN(Renin) ELISA Kit

Human REN(Renin) ELISA Kit
UniProt ID	P00797
Detection method	Sandwich
Standard	1000pg/mL
Sample type	Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL
Reaction Duration	3.5 hours
Alternative Names	REN1, HNFJ2, renin-1
Research Area	Cytokine;Tumor immunity;Infection immunity;
Test principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human REN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human REN. You can calculate the concentration of Human REN in the samples by comparing the OD of the samples to the standard curve.

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