| Human REN(Renin) ELISA Kit | |
| UniProt ID | P00797 |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Detection Method | Sandwich ELISA, Double Antibody |
| Detection Wavelength | OD450 |
| Reaction Duration | 4 hours |
| Storage | 2-8°C(Sealed), Don't cryopreserve. |
| Specificity | Specifically binds with REN , no obvious cross reaction with other analogues. |
A-1/264A Paschim Vihar, New Delhi - 110063
Human REN(Renin) ELISA Kit
| SKU Code | LBEK-093H |
| Species | Human |
| Sensitivity | 18.75pg/ml |
| Detection-range | 31.25-2000pg/ml |
| Size | 96 T | 48 T |
| Price | POR |
| Brand | Link Biotech |
- Product Specification
- Additional information
- Standard Curve
- Recovery
- Linearity
- Precision(%)
- Stability
Additional information
| sensitivity | 18.75pg/ml |
|---|---|
| detection-range | 31.25-2000pg/ml |
| Brand | Link Biotech |
- This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(pg/ml) OD-1 OD-2 Average 0 0.084 0.088 0.086 31.25 0.128 0.135 0.131 62.5 0.211 0.222 0.215 125 0.301 0.317 0.308 250 0.489 0.514 0.499 500 0.787 0.827 0.803 1000 1.221 1.283 1.246 2000 1.991 2.093 2.032 
Add a certain amount of REN into the sample. Calculate the recovery by comparing the measured value with the expected amount of REN in the sample.
| Sample Type | Recovery Range(%) | Average(%) |
|---|---|---|
| serum(n=10) | 97-101 | 100 |
| EDTA plasma(n=10) | 95-102 | 99 |
| Heparin plasma(n=10) | 96-102 | 99 |
Dilute the sample with a certain amount of REN at 1:2, 1:4 and 1:8 to get the recovery range.
| Sample Type | 1:2 | 1:4 | 1:8 |
|---|---|---|---|
| serum(n=10) | 91-105% | 90-97% | 92-99% |
| EDTA plasma(n=10) | 90-105% | 90-98% | 91-96% |
| Heparin plasma(n=10) | 91-104% | 89-94% | 90-99% |
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
| Item | Intra-assay Precision | Inter-assay Precision | ||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/ml) | 59.17 | 241.4 | 970.7 | 59.65 | 250.1 | 983.8 |
| Standard deviation | 2.69 | 13.3 | 53.97 | 3.33 | 14.53 | 57.85 |
| CV(%) | 4.55 | 5.51 | 5.56 | 5.59 | 5.81 | 5.88 |
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
| ELISA kit(n=5) | 37°C for 1 month | 2-8°C for 6 months | 2-8°C for 12 months |
| Average(%) | 80 | 95-100 | 85-98 |