| Human IL-6(Interleukin 6) ELISA Kit - Details | |
| Alternative Names | Interleukin-6 ELISA Kit, IL-6 ELISA Kit, B-cell stimulatory factor 2 ELISA Kit, BSF-2 ELISA Kit, CTL differentiation factor ELISA Kit, CDF ELISA Kit, Hybridoma growth factor ELISA Kit, Interferon beta-2 ELISA Kit, IFN-beta-2 ELISA Kit, IL6 ELISA Kit, IFNB2 ELISA Kit |
| Detection Wavelength | OD450 |
| Detection Method | Sandwich ELISA, Double Antibody |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Reaction Duration | 4 hours |
| Storage | 2-8°C(Sealed), Don't cryopreserve. |
| Specificity | Specifically binds with IL-6 , no obvious cross reaction with other analogues. |
| UniProt ID | P05231 |
Human IL-6(Interleukin 6) Elisa Kit
| SKU Code | LBFN-046H |
| Species | Human |
| Sensitivity | 2.813pg/ml |
| Detection Range | 4.688-300pg/ml (1pg=110mIU) |
| Size | 96 T | 48 T |
| Price | POR |
Additional information
| Sensitivity | 2.813pg/ml |
|---|---|
| Detection Range | 4.688-300pg/ml (1pg=110mIU) |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-6 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-6 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
Capture antibody and detection antibody by WB KO validation (Detect IL-6 KO A549 cells)