| Human IL-10(Interleukin 10) ELISA Kit | |
| Uniprot ID | P22301 |
| Detection Wavelength | OD450 |
| Detection Method | Sandwich ELISA, Double Antibody |
| Sample type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Reaction time | 4 hours |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Alternative Names | Interleukin-10 ELISA Kit, IL-10 ELISA Kit, Cytokine synthesis inhibitory factor ELISA Kit, CSIF ELISA Kit, IL10 ELISA Kit |
| Test Principle | This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-10 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-10 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-10 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
A-1/264A Paschim Vihar, New Delhi - 110063
Human IL-10(Interleukin 10) ELISA Kit
| SKU Code | LBEK-007H |
| Species | Human |
| Sensitivity | 4.688pg/ml |
| Detection Range | 7.813-500pg/ml (1ng=14500mIU) |
| Size | 96 T | 48 T |
| Price | POR |
Additional information
| Sensitivity | 4.688pg/ml |
|---|---|
| Detection-Range | 7.813-500pg/ml |
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