| Human Anti-Nuclear Antibody ELISA Kit - Details | |
| Alternative Names | ANA ELISA Kit, Nuclear antibody ELISA Kit |
| Detection Wavelength | OD450 |
| Detection Method | Indirect ELISA |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Reaction Duration | 3 hours |
| Storage | 2-8°C(Sealed), Don't cryopreserve. |
| Specificity | Specifically binds with anti-nuclear antibody , no obvious cross reaction with other analogues. |
Human Anti-Nuclear Antibody Elisa Kit
| SKU Code | LBFN-042H |
| Species | Human |
| Sensitivity | 0.938ng/ml |
| Detection Range | 1.563-100ng/ml |
| Size | 96 T | 48 T |
| Price | POR |
Additional information
| Sensitivity | 0.938ng/ml |
|---|---|
| Detection Range | 1.563-100ng/ml |
This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with anti-nuclear antibody bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-nuclear antibody in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.