Link Biotech
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Human Anti-Denosumab Antibody Elisa Kit

SKU Code LBFN-034H
Species Human
Sensitivity Qualitative
Detection Range Qualitative
Size 96 T | 48 T
Price POR
SKU: LBFN-034H
Human Anti-Denosumab Antibody ELISA kit - Details
Alternative Names Anti-Denosumab-Ab ELISA Kit
Detection Wavelength OD450
Detection Method Sandwich ELISA, Double Antigen
Application This immunoassay kit allows for the qualitative determination of anti-Denosumab antibodyinhuman serum or plasma.
Reaction Duration 4 hours
Storage 2-8°C(Sealed), Don't cryopreserve.
Specificity Specifically binds with Anti-Denosumab-Ab , no obvious cross reaction with other analogues.

Additional information

Sensitivity

Qualitative

Detection Range

Qualitative

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Denosumab was pre- coated onto 96-well plates. The test samples and HRP conjugated Denosumab were added to the wells, if there were any anti-Denosumab antibody in the samples, it would form a Denosumab – anti-Denosumab antibody – HRP- Denosumab complex. TMB substrates were used to visualize HRP enzymatic reaction. It was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.