Goat Leishmania Antibody (Leishmania-Ab)ELISA Kit

The qualitative detection of Goat Leishmania-Ab is based on the ELISA (Enzyme-Linked Immunosorbent Assay) technique. The microplate wells are pre-coated with Goat Leishmania-specific antigen. The corresponding antibodies in the sample bind the immobilized antigen after incubation. After washing the microplate wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled antigen conjugate specific to Goat Leishmania is added, which binds the captured antibodies, forming antigen-antibody-enzyme-labeled antigen complex upon incubation. In a second wash step, the unbound conjugate is removed.  The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue color reaction in wells containing the complex. A stop solution containing acid terminates the reaction, producing a yellow end-point coloration proportional to the amount of specific antibodies present in the sample. Absorbance is measured at 450 nm using an ELISA microwell plate reader. Analysis is completed by comparing the OD values of samples to a cut-off value.