Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

General Vitamin B1 (VB1)ELISA Kit

SKU Code LBEK-0025G
Species General
Sensitivity 0.1ng/ml
Detection Range 0.6ng/ml-40ng/ml
Size 96 T | 48 T
Price POR
SKU: LBEK-0025G
General Vitamin B1 (VB1)ELISA Kit
Wavelength 450nm
Sample Volume 10ul
Standard 54ng/ml
Intended Use Enzyme immunoassay for the quantitative detection of General VB1 antigen in serum, plasma, culture media, or any biological fluid.
Purpose Our General Vitamin B1 (VB1)ELISA kit is to assay VB1 levels in General serum, plasma, culture media or any biological fluid.

Additional information

sensitivity

0.1ng/ml

detection-range

0.6ng/ml-40ng/ml

Brand

Link Biotech

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to VB1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for VB1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain VB1 and HRP conjugated VB1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of VB1. You can calculate the concentration of VB1 in the samples by comparing the OD of the samples to the standard curve.