General THB/ Tetrahydrobiopterin ELISA Kit

SKU Code	LBEK-0086G
Species	General
Sensitivity	0.067ng/ml
Detection Range	0.156-10ng/ml
Size	96 T \| 48 T
Price	POR

General THB/ Tetrahydrobiopterin ELISA Kit
Method	Competitive
Samples	In serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Intended use	This immunoassay kit allows for the in vitro quantitative determination of General THB concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tetrahydrobiopterin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	97-107%	111-119%	99-109%	99-108%
EDTA plasma(n=5)	103-116%	82-93%	101-101%	107-117%
heparin plasma(n=5)	111-123%	103-113%	115-127%	100-110%

Additional information

sensitivity	0.067ng/ml
detection-range	0.156-10ng/ml
brand	Link Biotech

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General THB, During the reaction, General THB in the sample or standard competes with a fixed amount of biotin-labeled General THB for sites on a pre-coated Monoclonal antibody specific to General THB. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General THB in the samples is then determined by comparing the O.D. of the samples to the standard curve.