General 8-Oxo-2′-deoxyguanosine ,8OHDG elisa kit

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General 8OHDG, During the reaction, General 8OHDG in the sample or standard competes with a fixed amount of biotin-labeled General 8OHDG for sites on a pre-coated Monoclonal antibody specific to General 8OHDG. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General 8OHDG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-Oxo-2′-deoxyguanosine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

General Annotation

Function:

8-Oxo-2′-deoxyguanosine (8-oxo-dG) is an oxidized derivative of deoxyguanosine. 8-oxo-dG is one of the major products of DNA oxidation. Concentrations of 8-oxo-dG within a cell are a measurement of oxidative stress.