Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Corticosterone and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
1:4 |
1:8 |
1:16 |
|
84-93% |
103-113% |
94-103% |
|
111-120% |
96-106% |
90-99% |
|
heparin plasma(n=5) |
111-121% |
110-120% |
107-116% |
105-113% |
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General CORTI, During the reaction, General CORTI in the sample or standard competes with a fixed amount of biotin-labeled General CORTI for sites on a pre-coated Monoclonal antibody specific to General CORTI. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General CORTI in the samples is then determined by comparing the O.D. of the samples to the standard curve.
General Annotation
Function:
In many species, including amphibians, reptiles, rodents and birds, corticosterone is a main glucocorticoid, involved in regulation of fuel, immune reactions, and stress responses. However, in humans, corticosterone is produced primarily in the zona fasciculata of the adrenal cortex. It has only weak glucocorticoid and mineralocorticoid potencies in humans and is important mainly as an intermediate in the steroidogenic pathway from pregnenolone to aldosterone. Corticosterone is converted to aldosterone by aldosterone synthase, found only in the mitochondria of glomerulosa cells. Glomerulosa cells are found in the Zona glomerulosa, which is the most superficial region of endocrine cells in the adrenal cortex.
Location:
Corticosterone (CORT) is a 21-carbon steroid hormone of the corticosteroid type produced in the cortex of the adrenal glands.
