General Chlamydia Antibody (Chlamydia-Ab)ELISA Kit

The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an antigen specific to Chlamydia-Ab, make it to solid-phase antigen.Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for Chlamydia-Ab is added to each Microplate well and incubated,so the antigen–antibody-Enzyme labeled antigen complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain Chlamydia-Ab and HRP conjugated Chlamydia antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of Chlamydia-Ab is determined by comparing with the CUT OFF value.