General Calcitriol ELISA Kit

SKU Code	LBEK-0088G
Species	General
Sensitivity	0.054ng/mL
Detection Range	0.156-10 ng/mL
Size	96 T \| 48 T
Price	POR

General Calcitriol ELISA Kit
Method	Competitive
Samples	In serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Intended use	This immunoassay kit allows for the in vitro quantitative determination of General Calcitriol concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 8.6%

Recovery

Recovery was determined by spiking various levels of Calcitriol into serum and plasma.

Recovery was determined by spiking various levels of Calcitriol into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	90	88-98
Plasma	84	79-93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcitriol and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	110-119%	100-110%	100-110%	108-117%
EDTA plasma(n=5)	114-123%	91-101%	85-97%	107-117%
heparin plasma(n=5)	107-116%	105-115%	100-110%	103-113%

Additional information

sensitivity	0.054ng/mL
detection-range	0.156-10 ng/mL
brand	Link Biotech

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General Calcitriol, During the reaction, General Calcitriol in the sample or standard competes with a fixed amount of biotin-labeled General Calcitriol for sites on a pre-coated Monoclonal antibody specific to General. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General Calcitriol in the samples is then determined by comparing the O.D. of the samples to the standard curve.