| General Folic Acid (FA)ELISA Kit | |
| Method | Competitive |
| Standard | 18ng/ml |
| Sample Volume | 10ul |
| Purpose | Our General Folic Acid (FA)ELISA kit is to assay FA levels in General serum, plasma, culture media or any biological fluid. |
A-1/264A Paschim Vihar, New Delhi - 110063
General ADMA/ Asymmetric dimethylarginine ELISA Kit
| SKU Code | LBEK-021G |
| Species | General |
| Sensitivity | 5.4ng/mL |
| Detection Range | 15.6-1000ng/mL |
| Size | 96 T | 48 T |
| Price | POR |
Additional information
| sensitivity | 5.4ng/mL |
|---|---|
| detection-range | 15.6-1000ng/mL |
| brand | Link Biotech |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Asymmetric dimethylarginine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1：2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 103-113% | 104-113% | 109-119% | 93-102% |
| EDTA plasma(n=5) | 104-114% | 90-100% | 83-92% | 99-109% |
| heparin plasma(n=5) | 93-103% | 89-97% | 96-104% | 102-112% |
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General ADMA, During the reaction, General ADMA in the sample or standard competes with a fixed amount of biotin-labeled General ADMA for sites on a pre-coated Monoclonal antibody specific to General ADMA. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General ADMA in the samples is then determined by comparing the O.D. of the samples to the standard curve.