Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

FS-Rat sCD86 ELISA Kit

SKU Code LBEK-008R
Species Rat
Sensitivity 8.93 pg/mL
Detection Range 15.63-1000pg/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-008R
Categories: ,
FS-Rat sCD86(Soluble Cluster of Differentiation 86) ELISA Kit
Uniprot ID O35531
Detection Method Sandwich
Standard 1000pg/mL
Reaction time 1.5hours
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area Signaling transduction, Cancer;
Alternative Names Activation B7-2 antigen, B70, B7-2 antigen, B72, B7-2, B-lymphocyte activation antigen B7-2, BU63, CD28 antigen ligand 2, CD28LG2B7-2 antigen), CD86 antigen, CD86 molecule, CD86, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat sCD86. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat sCD86 are added to the micro ELISA plate wells. Rat sCD86 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat sCD86. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Rat sCD86. The concentration of Rat sCD86 in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

8.93 pg/mL

detection-range

15.63-1000pg/mL

Brand

Link Biotech