Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

FS-Rat ICAM-1/CD54 ELISA Kit

SKU Code LBEK-011R
Species Rat
Sensitivity 0.01 ng/mL
Detection Range 0.31-20ng/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-011R
Categories: ,
FS-Rat ICAM-1/CD54(Intercellular Adhesion Molecule 1) ELISA Kit
Uniprot ID Q00238
Detection Method Sandwich
Standard 20ng/mL
Reaction time 1.5hours
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area Signaling transduction, Metabolism ;
Alternative Names BB2, CD54 antigen, CD54, cell surface glycoprotein P3.58, human rhinovirus receptor, ICAM1, ICAM-1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, intercellular adhesion molecule 1, Major group rhinovirus receptor, P3.58
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ICAM-1/CD54. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ICAM-1/CD54 are added to the micro ELISA plate wells. Rat ICAM-1/CD54 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ICAM-1/CD54. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Rat ICAM-1/CD54. The concentration of Rat ICAM-1/CD54 in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

0.01 ng/mL

detection-range

0.31-20ng/mL

brand

Link Biotech