FS-Rat BMG/β2-MG ELISA Kit

FS-Rat BMG/β2-MG(Beta-2-Microglobulin) ELISA Kit
Uniprot ID	P07151
Detection Method	Sandwich
Standard	2000pg/mL
Reaction time	1.5hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Cancer, Immunology;
Alternative Names	B2M, beta 2Microglobulin, beta 2-Microglobulin, beta chain of MHC class I molecules, beta-2-microglobin, beta-2-microglobulin
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BMG/β2-MG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat BMG/β2-MG are added to the micro ELISA plate wells. Rat BMG/β2-MG in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat BMG/β2-MG. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Rat BMG/β2-MG. The concentration of Rat BMG/β2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information