FS-Mouse F12 ELISA Kit

FS-Mouse F12(Coagulation Factor Ⅻ) ELISA Kit
Uniprot ID	Q80YC5
Detection Method	Sandwich
Standard	4000pg/mL
Reaction time	1.5hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Cancer, Immunology;
Alternative Names	Hageman factor,F12, HAE3, HAEX, HAF
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse F12. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse F12 are added to the micro ELISA plate wells. Mouse F12 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse F12. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse F12. The concentration of Mouse F12 in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information