Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

FS-Human NGAL ELISA Kit

SKU Code LBEK-0038H
Species Human
Sensitivity 0.16 ng/mL
Detection Range 0.31-20ng/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-0038H
FS-Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
Uniprot ID P80188
Detection Method Sandwich
Standard 20ng/mL
Reaction time 1.5hours
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area Signaling transduction, Metabolism ;
Alternative Names 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, LCN2, lipocalin 2 (oncogene 24p3), lipocalin 2, Lipocalin2, Lipocalin-2, migration-stimulating factor inhibitor, MSFI, neutrophil gelatinase-associated lipocalin, NGAL, NGALlipocalin-2, Oncogene 24p3, p25, Siderocalin, Uterocalin
Test Principle This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGAL. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human NGAL are added to the micro ELISA plate wells. Human NGAL in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human NGAL. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human NGAL. The concentration of Human NGAL in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

0.16 ng/mL

detection-range

0.31-20ng/mL

brand

Link Biotech