FS-Human MCP-1 ELISA Kit

FS-Human MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit
Uniprot ID	P13500
Detection Method	Sandwich
Standard	1000pg/mL
Reaction time	1.5hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Cancer, Immunology;
Alternative Names	C-C motif chemokine ligand 2, CCL2, GDCF-2, HC11, HSMCR30, MCAF, Mcp1, MCP-1, SCYA2, SMC-CF
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human MCP-1 are added to the micro ELISA plate wells. Human MCP-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human MCP-1. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human MCP-1. The concentration of Human MCP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information