FS-Human C-P ELISA Kit

FS-Human C-P(C-Peptide) ELISA Kit
Uniprot ID	P01308
Detection Method	Sandwich
Standard	2000pg/mL
Reaction time	1.5hours
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area	Neuroscience, Signaling transduction, Cancer;
Alternative Names	C-P, C-Peptide, IDDM2, ILPR, Insulin C-Peptide, insulin, IRDN, MODY10, Proinsulin C-Peptide, proinsulin
Test Principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C-P. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human C-P are added to the micro ELISA plate wells. Human C-P in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human C-P. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human C-P. The concentration of Human C-P in the samples is then determined by comparing the OD of the samples to the standard curve.

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