Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

FS-Cor(Cortisol) ELISA Kit

SKU Code LBEK-0012U
Species Universal
Sensitivity 0.17 ng/mL
Detection Range 0.31-20ng/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-0012U
FS-Cor(Cortisol) ELISA Kit
Uniprot ID N/A
Detection Method Competitive
Standard 20ng/mL
Reaction time 45min
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=50μL
Research Area Cardiovascular, Cell biology, Neuroscience, Cancer, Immunology, Metabolism
Alternative Names Compound F, Cortisol, Hydrocortisone
Test Principle This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cor. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Cor. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cor in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information

sensitivity

0.17 ng/mL

detection-range

0.31-20ng/mL

brand

Link Biotech