| E.coli Host Cell Protein ELISA Kit(96T) | |
| Storage | 2-8℃ |
| LOD | 1.95 ng/ml |
| CV of Inter plate | 8.1-11.5% |
| CV of Intra plate | 7.3-13.1% |
| LOQ | 3.9 ng/ml |
| Appliction | The kit is intended for the quantitative determination of residual host cell protein in protein therapeutics expressed by E. coli expression systems. |
A-1/264A Paschim Vihar, New Delhi - 110063
E.coli Host Cell Protein ELISA Kit(96T)
| SKU Code | LBEK-001P |
| Species | HCP Residues |
| Sensitivity | N/A |
| Detection Range | N/A |
| Size | 96 T | 48 T |
| Price | POR |
Additional information
| sensitivity | – |
|---|---|
| detection-range | – |
| brand | Link Biotech |
Escherichia coli, commonly known as E. coli, is widely utilized as an expression system. During the process of extracting target proteins from fragmented bacterial cells, a substantial portion of host proteins is released along with the target protein. These host proteins exhibit strong immunogenicity, which can lead to adverse toxicity or immune reactions, jeopardizing product safety and quality, and causing potential biological contamination. One of the aims of downstream processes in the production of biological medicinal products is to remove these potential hazards.
Therefore, it is essential to minimize the residual levels of host cell proteins (HCP), and in the downstream purification process development, a scientifically sound method for determining the concentration of HCP in finished or semi-finished products is necessary. Enzyme-linked immunosorbent assay (ELISA) has a high sensitivity and is therefore designated as the gold standard for HCP detection by regulatory agencies.
MATERIALS (Note: Storage at 2-8℃)**
| Reagents | Specification | Quantity | |
| 1 | Pre-Coated Microplate
(Detachable) |
96 wells | 1 plate
(Keep Sealed) |
| 2 | Standard (Stock Solution) -500ug/ml | 50ul | 1 tube |
| 3 | Detection antibody (100×) | 150ul | 1 tube |
| 4 | TMB Substrates | 10ml | 1 vial (Avoid Light) |
| 5 | Stop Solution | 10ml | 1 vial |
| 6 | Wash Solution (100×) | 10ml | 1 vial |
| 7 | Diluent Buffer (10×) | 10ml | 1 vial |
| 8 | Plate Sealer | 4 pieces | |
| 9 | Instruction Manual | 1 |
