| E.coli HCPs (E.coli Host Cell Proteins) ELISA Kitt - Details | |
| Alternative Names | E.coli HCPs ELISA Kit, E.coli host cell proteins ELISA Kit |
| Sample Type | Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Detection Wavelength | OD450 |
| Detection Method | Sandwich ELISA, Double Antibody |
| Reaction Duration | 4 hours |
| Storage | 2-8°C(Sealed), Don't cryopreserve. |
| Specificity | Specifically binds with E.coli HCPs , no obvious cross reaction with other analogues. |
E-mail: support@linkbiotech.co.in
Website: www.linkbiotech.co.in
Website: www.linkbiotech.co.in
E.coli HCPs Elisa Kit
| SKU Code | LBFN-030U |
| Species | Universal |
| Sensitivity | 1.875ng/ml |
| Detection Range | 3.125-200ng/ml |
| Size | 96 T | 48 T |
| Price | POR |
Additional information
| Sensitivity | 1.875ng/ml |
|---|---|
| Detection Range | 3.125-200ng/ml |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti E.coli HCPs antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with E.coli HCPs bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of E.coli HCPs in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.