Cyclic Adenosine Monophosphate ELISA Kit

SKU Code	LBRB-027U
Species	Universal
Sensitivity	92.5 pg/mL
Detection Range	312.5-20000 pg/mL
Size	96 T \| 48 T
Price	$ 365 \| $ 265

Description

cAMP(Cyclic Adenosine Monophosphate) ELISA Kit - Product Details
Alternative Names	c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate
Uniprot ID	N/A
Standard	20000 pg/mL
Detection method	Competitive
Sample type	Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids
Reaction time	1.5H
Research Area	Signal transduction;Metabolic pathway
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to cAMP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.

Additional information

Sensitivity	92.5 pg/mL
Detection Range	312.5-20000 pg/mL

Technical Data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

(pg/mL)	OD
20000.00	0.195
10000.00	0.331
5000.00	0.546
2500.00	0.880
1250.00	1.308
625.00	1.700
312.50	2.076
0.00	2.516

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/mL)	393.70	840.34	5617.98	409.84	628.93	6993.01
Standard deviation	41.95	92.71	290.18	52.22	115.40	361.21
C V (%)	5.59	5.81	3.60	6.11	5.77	2.93

Rate of Recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Avg Recovery(%)
Serum (n=8)	80-92	86
EDTA plasma (n=8)	90-105	97
Cell culture media (n=8)	88-102	95

Linear

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	85-94	86-97	87-98
1:2	Average (%)	91	94	93
1:4	Range (%)	87-99	86-97	85-92
1:4	Average (%)	87	90	86
1:8	Range (%)	85-101	88-95	80-96
1:8	Average (%)	96	93	91
1:16	Range (%)	80-90	92-101	89-100
1:16	Average (%)	89	101	97

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