| COVID-19 nucleoprotein ELISA Kit - Details | |
| Alternative Names | SARS-CoV-2 nucleoprotein ELISA Kit |
| Detection Wavelength | OD450 |
| Detection Method | Sandwich ELISA, Double Antibody |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Reaction Duration | 4 Hours |
| Storage | 2-8°C(Sealed), Don't cryopreserve. |
| Specificity | Specifically binds with COVID-19 nucleoprotein , no obvious cross reaction with other analogues. |
| UniProt ID | P0DTC9 |
COVID-19 Nucleoprotein Elisa Kit
| SKU Code | LBFN-062U |
| Species | Universal |
| Sensitivity | 37.5pg/ml |
| Detection Range | 62.5-4000pg/ml |
| Size | 96 T | 48 T |
| Price | POR |
Additional information
| Sensitivity | 37.5pg/ml |
|---|---|
| Detection-Range | 62.5-4000pg/ml |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti COVID-19 nucleoprotein antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with COVID-19 nucleoprotein bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of COVID-19 nucleoprotein in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
