| Chicken CORT(Corticosterone) ELISA Kit - Details | |
| Alternative Names | Corticosterone ELISA Kit, CORT ELISA Kit |
| Detection Wavelength | OD450 |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Reaction Duration | 2 Hours |
| Storage | 2-8°C(Sealed), Don't cryopreserve. |
| Specificity | Specifically binds with CORT , no obvious cross reaction with other analogues. |
Chicken CORT(Corticosterone) Elisa Kit
| SKU Code | LBFN-055C |
| Species | Chicken |
| Sensitivity | 1.688ng/ml |
| Detection Range | 2.813-180ng/ml |
| Size | 96 T | 48 T |
| Price | POR |
Additional information
| Sensitivity | 1.688ng/ml |
|---|---|
| Detection-Range | 2.813-180ng/ml |
This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with CORT. CORT in the sample or standard competes with a fixed amount of biotin-labeled CORT for sites on a pre-coated antibody specific to CORT. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of CORT in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
