Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

Canine Leptospira IgM (LEP-IgM)ELISA Kit

SKU Code LBEK-011CA
Species Canine
Sensitivity N/A
Detection-range N/A
Size 96 T | 48 T
Price POR
Brand Link Biotech
SKU: LBEK-011CA
Canine Leptospira IgM (LEP-IgM)ELISA Kit
Sample Volume 10ul
Wavelength 450nm
Qualitative Negative/Positive
Purpose Our Canine Leptospira IgM (LEP-IgM)ELISA Kit is to for the qualitative determination of LEP-IgM in Canine serum, plasma, culture media or any biological fluid.

Additional information

sensitivity

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detection-range

–

Brand

Link Biotech

The ELISA is based on the the qualitative enzyme immunoassay technique.The Microplate provided in this kit has been pre-coated with an antigen specific to LEP-IgM, make it to solid-phase antigen.Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for LEP-IgM is added to each Microplate well and incubated,so the antigen-antibody-Enzyme labeled antigen complex is formed.Following a wash to remove any unbound reagent,then the TMB substrate solution is added to each well. Only those wells that contain LEP-IgM and HRP conjugated LEP antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of LEP-IgM is determined by comparing with the CUT OFF value.