| Bovine HP (Haptoglobin) ELISA Kit - Details | |
| Alternative Names | Haptoglobin ELISA Kit, Zonulin ELISA Kit, Haptoglobin alpha chain ELISA Kit, Haptoglobin beta chain ELISA Kit, HP ELISA Kit |
| Detection Wavelength | OD450 |
| Detection Method | Sandwich ELISA, Double Antibody |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Reaction Duration | 4 Hours |
| Storage | 2-8°C(Sealed), Don't cryopreserve. |
| Specificity | Specifically binds with HP , no obvious cross reaction with other analogues. |
| UniProt ID | Q2TBU0 |
Bovine HP (Haptoglobin) Elisa Kit
| SKU Code | LBFN-059B |
| Species | Bovine |
| Sensitivity | 0.469ug/ml |
| Detection Range | 0.781-50ug/ml |
| Size | 96 T | 48 T |
| Price | POR |
Additional information
| Sensitivity | 0.469ug/ml |
|---|---|
| Detection-Range | 0.781-50ug/ml |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with HP bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of HP in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
