Description
| Alternative Names: | 3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine | |||||||||||||||||||||||||||
| Catalogue No. | 6687ELK | |||||||||||||||||||||||||||
| Size | 96T | |||||||||||||||||||||||||||
| Reactivity | General | |||||||||||||||||||||||||||
| Range | 4.69-300 ng/mL | |||||||||||||||||||||||||||
| Sensitivity | 1.35 ng/mL | |||||||||||||||||||||||||||
| Assay Type | Competitive Inhibition | |||||||||||||||||||||||||||
| Sample Type | serum, plasma and other biological fluids | |||||||||||||||||||||||||||
| Assay Length | 2h | |||||||||||||||||||||||||||
| Research Area | Metabolic pathway; Infection immunity; Rheumatology; | |||||||||||||||||||||||||||
| Test principle | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Nitrotyrosine(NT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nitrotyrosine(NT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitrotyrosine(NT) in the samples is then determined by comparing the OD of the samples to the standard curve. | |||||||||||||||||||||||||||
| Standard Curve | 
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| Precision | Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. |
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| Recovery | Matrices listed below were spiked with certain level of recombinant 3-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 3-NT in samples.
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| Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| For Research Use Only |
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