Haemophilus parasuis Antibody (HPs-Ab)ELISA Kit

The ELISA is based on the the qualitative enzyme immunoassay technique. The Microplate provided in this kit has been pre-coated with an antigen specific to HPs-Ab, make it to solid-phase antigen. Samples are added to the Microplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for HPs is added to each Microplate well and incubated, so the antigen-antibody-Enzyme labeled antigen complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain HPs-Ab and HRP conjugated HPs antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of HPs-Ab is determined by comparing with the CUTOFF value.