Guinea pig Endothelin 1 ,ET-1 ELISA Kit

The quantitative detection of Guinea pig ET-1 antigen is based on the ELISA
(Enzyme-Linked Immunosorbent Assay) technique. The microplate wells are pre-coated with
an antibody specific to Guinea pig ET-1. After incubation, the corresponding antigen in the
sample or standards will bind to the immobilized antibody. After washing the microplate
wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled antibody
conjugate is added, which binds to the antigen, forming antibody-antigen-enzyme-labeled
antibody complex upon incubation. In a second wash step, all unbound material is removed.
The immune complex formed by the bound conjugate is visualized by adding
Tetramethylbenzidine (TMB) substrate, which gives a blue color reaction in wells containing
the complex. A stop solution containing acid terminates the reaction, producing a yellow
end-point coloration proportional to the amount of specific antigen present in the sample.
Absorbance is measured at 450 nm using an ELISA microwell plate reader. Analysis is
completed by comparing the OD values of samples to the standard curve.