| Human ALD(Aldosterone) ELISA Kit | |
| UniProt ID | CAS: 52-39-1 |
| Sample Volume | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Storage | 2-8°C(Sealed), Don't cryopreserve. |
| Specificity | Specifically binds with ALD , no obvious cross reaction with other analogues. |
A-1/264A Paschim Vihar, New Delhi - 110063
Human ALD(Aldosterone) ELISA Kit
| SKU Code | LBEK-089H |
| Species | Human |
| Sensitivity | 1.875pg/ml |
| Detection-range | 3.125-200pg/ml |
| Size | 96 T | 48 T |
| Price | POR |
| Brand | Link Biotech |
Additional information
| sensitivity | 1.875pg/ml |
|---|---|
| detection-range | 3.125-200pg/ml |
| Brand | Link Biotech |
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
| STD.(pg/ml) | OD-1 | OD-2 | Average |
|---|---|---|---|
| 0 | 2.111 | 2.173 | 2.142 |
| 3.125 | 1.872 | 1.926 | 1.899 |
| 6.25 | 1.333 | 1.371 | 1.352 |
| 12.5 | 0.91 | 0.936 | 0.923 |
| 25 | 0.604 | 0.622 | 0.613 |
| 50 | 0.413 | 0.425 | 0.419 |
| 100 | 0.269 | 0.277 | 0.273 |
| 200 | 0.163 | 0.167 | 0.165 |
Add a certain amount of ALD into the sample. Calculate the recovery by comparing the measured value with the expected amount of ALD in the sample.
| Sample Type | Recovery Range(%) | Average(%) |
|---|---|---|
| serum(n=10) | 85-102 | 92 |
| EDTA plasma(n=10) | 88-103 | 92 |
| Heparin plasma(n=10) | 88-102 | 94 |
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
| Item | Intra-assay Precision | Inter-assay Precision | ||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/ml) | 6.52 | 24.96 | 98.62 | 5.91 | 24.36 | 98.03 |
| Standard deviation | 0.35 | 1.17 | 4.91 | 0.34 | 1.25 | 4.57 |
| CV(%) | 5.33 | 4.69 | 4.98 | 5.74 | 5.12 | 4.66 |
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
| ELISA kit(n=5) | 37°C for 1 month | 2-8°C for 6 months | 2-8°C for 12 months |
| Average(%) | 80 | 95-100 | 85-98 |