|
Samples |
In serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. |
|
Intended Use |
This immunoassay kit allows for the in vitro quantitative determination of General PGE2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prostaglandin E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
1:4 |
1:8 |
1:16 |
|
94-103% |
89-101% |
105-115% |
|
105-115% |
108-118% |
95-105% |
|
heparin plasma(n=5) |
90-102% |
94-103% |
94-104% |
96-107% |
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General PGE2, During the reaction, General PGE2 in the sample or standard competes with a fixed amount of biotin-labeled General PGE2 for sites on a pre-coated Monoclonal antibody specific to General. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General PGE2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
General Annotation
Function:
The naturally occurring prostaglandin E2 (PGE2) is known in medicine as dinoprostone. It has important effects in labour (softens cervix and causes uterine contraction) and also stimulates osteoblasts to release factors that stimulate bone resorption by osteoclasts. PGE2 is also the prostaglandin that ultimately induces fever.
Location:
Like other prostaglandins, dinoprostone can be used as an abortifacient. It is a direct vasodilator, relaxing smooth muscles, and it inhibits the release of noradrenaline from sympathetic nerve terminals. It does not inhibit platelet aggregation, where PGI2 does. It works by binding and activating the prostaglandin E2 receptor.
