Link Biotech

Link Biotech

A-1/264A Paschim Vihar, New Delhi - 110063

Elisa

General VAL/ Valine ELISA Kit

SKU Code LBEK-0019G
Species General
Sensitivity 0.34nmol/mL
Detection Range 1.56-100nmol/mL
Size 96 T | 48 T
Price POR
SKU: LBEK-0019G
Categories: ,
General VAL/ Valine ELISA Kit
Method Competitive
Sample Type In serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Intended use This immunoassay kit allows for the in vitro quantitative determination of General VAL concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Valine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1：2 1:4 1:8 1:16
serum(n=5) 93-103% 88-98% 97-107% 109-118%
EDTA plasma(n=5) 105-116% 107-119% 110-120% 106-118%
heparin plasma(n=5) 84-93%  88-96% 101-111% 91-101%

Additional information

sensitivity

0.34nmol/mL
detection-range

1.56-100nmol/mL
brand

Link Biotech

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to General VAL, During the reaction, General VAL in the sample or standard competes with a fixed amount of biotin-labeled General VAL for sites on a pre-coated Monoclonal antibody specific to General VAL. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of General VAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.